ABOUT

The central mammalian cell culture facility that supports the research work of the whole institute is equipped with high end instruments like Biosafety cabinets (Class II), CO2 incubators, Hypoxia workstation, fully automated Fluorescence microscopes with live cell imaging facility, Flow cytometer with sorter, electroporator, refrigerators/freezers, centrifuges, water bath, automated cell counter and a liquid nitrogen storage tank.

The facility also has a separate space for primary cell culture which includes Mechanical Bioreactor and tissue dissociator. The cell line repository consists of mammalian cancer cell lines, murine primary cell lines and noncancerous cell lines.

The facility is used by more than 50 PhD scholars and MS students from across the departments for their project work. All the students are provided with an induction training which focuses on demonstration of basic cell culture techniques and good lab practices.

The current research activities include neuronal studies, NGS, transcriptomics, cancer biology studies, diabetic nephropathy, proteomics, metabolomics in cancer, 3D cell culture, slice culture, drug screening, biomaterial screening etc.

Our Cell Culture Facility is committed to developing practical skills and strengthening technical expertise in mammalian cell culture and related laboratory techniques. Through structured skill development and hands-on training programs, the facility provides students, researchers, academicians, and biotechnology professionals with an opportunity to gain practical exposure to modern cell culture methodologies.

Equipment List

1. Biosafety Cabinet (Thermo A2 1300 series, Imset BSCA2)
2. CO2 Incubator (Haier HCP 168, Thermo Forma Stericycle)
3. Fluorescence Microscope (Zeiss Axio Observer A1, Zeiss Axio Observer Z1)
4. Bright Field Microscope (Leica DMi1)
5. Centrifuge (Thermo Sorvall Legend, Micro 17)
6. Refrigerator (Thermo, Vestfrost)
7. Deep Freezer -30 (Thermo FDE series)
8. Hypoxia Chamber (Don Whitley H35)
9. FACS (Biorad S3e)
10. Analytical Balance (OHAUS)
11. Liquid Nitrogen Tank (Thermo 1368, NEB Cryosalvum 3600)
12. Waterbath (Julabo pura10)

Facility

Cell Culture Room 1	Cell Culture Room 2
Cell Culture Room 3	Primary Culture Room

Flow Cytometry Room

Skill Development Programs/Workshops Organized

1. The Principles and Techniques of Mammalian Cell Culture (September 2026)

2. Microphysiological Systems Bootcamp, Next Generation Biomaterials and Techniques in 3D Cell Culture (March 2025)