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National Institute of Pharmaceutical Education and Research (NIPER) - Ahmedabad

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Siddheshwar

Dr. Siddheshwar Chauthe, Ph.D

Assistant Professor , Department of Natural Products

Research Interest

  • Bioassay-guided isolation, structure elucidation and synthetic modification of bioactive compounds from medicinal plants.
  • Development of analytical methods for standardization of polyherbal formulations using hyphenated techniques.
  • To establish Q-markers for the quality standards of traditional Ayurvedic /herbal medicines.

Experience

  • March 2020 -Present
    Assistant Professor

    NIPER- Ahmedabad, Palaj, Gandhinagar

  • October 2019-March 2020
    Senior Research Investigator (Team Lead LCMS and HPLC Sections)

    Biocon-BMS R&D Center (BBRC), Syngene International Ltd, Bangalore

  • April 2016-September 2019
    Research Investigator (Team Lead LCMS and HPLC Sections)

    Biocon-BMS R&D Center (BBRC), Syngene International Ltd, Bangalore

  • March 2014- Nov 2015
    Research Specialist-II (Post Doc)

    Metabolomics Center, Georgetown University Medical Center, USA

  • June 2012 - March 2014
    Assistant Manager

    Pharmaceutical R&D, GlaxoSmithKline, Nashik, India

  • March 2005 - Aug 2006
    Research Associate

    R & D, Piramal Life Sciences, Limited, Mumbai

  • May 2004 - Dec 2005
    R&D Officer

    R & D, Sami Labs Limited, Bangalore

Honours

  • BBRC Bravo Award for developing LCMS based preparative HPLC method development approach (2016).
  • NIH Common Fund Metabolomics Initiative: $1000 travel award to attend the 2nd Annual UAB Workshop on Metabolomics, June 2nd – 5th, 2014.
  • Three GlaxosmithKline Bravo awards for successfully driving various R & D projects (2012-13).
  • Won $8800 Innocentive award for solving the Innocentive challenge entitled “Process Mass Intensities.” Sponsored by Merck Pharmaceutical Inc, 2010.
  • Won $1000 Innocentive award for solving the Innocentive challenge entitled “Novel Sources for Extraction of Xanthophyll Carotenoids”(2010).
  • Senior Research Fellowship, Council of scientific and industrial research (CSIR), Ministry of Science and Technology, India (2010-12).
  • Senior Research Fellowship, Department of biotechnology (DBT), Ministry of Science and Technology, India (2008-2010).
  • Junior Research Fellowship, Department of biotechnology (DBT), Ministry of Science and Technology, India (2006-2008).
  • Master program Fellowship, Graduate aptitude test in engineering (GATE) conducted by IIT Madras (2002).

Publications

  • Teja, P. K.; Ly, B. Q.; Shah, D. K and Chauthe, S. K. (2023) Isolation, Characterization of Undescribed Alkaloids, and Semisynthetic Modifications of Cytotoxic Pyranoacridone Alkaloids from Glycosmis pentaphylla. ACS Omega, 2023, Article in press. https://doi.org/10.1021/acsomega.2c08100.
  • Kumar, S.; Gajjela, R.; Kumar, H.; Arulraj, R. A.; Subramaniam, S.; Kothandaramachandran, T.; Sudhir V, S.; Chauthe, S. K.; Gupta, A.; Mathur, A.; Roy, A.; Bagadi, M.; Caceres-Cortes, J. A Strategy for Evaluation of Isotopic Enrichment and Structural Integrity of Deuterium Labelled Compounds by Using HR-MS and NMR. Anal. Methods, 2023. https://doi.org/10.1039/D2AY01980A.
  • Arulraj, R. A.; Gajjela, R.; Chauthe, S. K.; Bagadi, M.; Mathur, A. High-Throughput GC-FID Method for the Determination of Residual Solvents in Early-Phase Drug Discovery Samples. Chromatographia 2022, 1–10.
  • Chauthe, S. K.; Sidduri, P.; Reddy, M.; Sistla, R.; Byri, V.; Uttaravalli, M. D.; Botlagunta, M.; Kumar, H.; Gupta, A.; Gupta, A. K.; Bajpai, L.; Bagadi, M.; Mathur, A. RP-HPLC Separation of Interconvertible Rotamers of a 5-Tetrahydroisoquinolin-6-Yl-Pyridin-3-Yl Acetic Acid Derivative and Confirmation by VT NMR and DFT Study. J. Pharm. Biomed. Anal. 2022, 212, 114675. https://doi.org/https://doi.org/10.1016/j.jpba.2022.114675.
  • Singh, D. K.; Chauthe, S.K.; Anjanappa, P.; Kumar, S.; Arulraj, R. A.; Gajjela, R.; Mohammed, N.; Kanthappa, V. T.; Karmakar, A.; Gupta, A. K.; Bajpai, L.; Bagadi, M.; Mathur, A. (2021) Synthesis and Characterization of a Series of N,N′-Substituted Urea Derivatives by Using Electrospray Ionization Tandem Mass Spectrometry: Differentiation of Positional Isomers. Rapid Commun. Mass Spectrom, 35 (19), 1–12. https://doi.org/10.1002/rcm.9161
  • Rajagopal, M. U.; Bansal, S.; Kaur, P.; Jain, S. K.; Altadil, T.; Hinzman, C. P.; Li, Y.; Moulton, J.; Singh, B.; Bansal, S.;Chauthe, S.K.; Singh, R.; Banerjee, P. P.; Mapstone, M.; Fiandaca, M. S.; Federoff, H. J.; Unger, K.; Smith, J. P.; Cheema, A. K. (2021) TGFβ Drives Metabolic Perturbations during Epithelial Mesenchymal Transition in Pancreatic Cancer: TGFβ Induced EMT in PDAC. Cancers, https://doi.org/10.3390/cancers13246204
  • Shyamsundar, M.; Chauthe, S.K.; Subramani, L.; Subbaiah, M. A. M.; Gupta, A.; Bajpai, L.; Bagadi, M.; Mathur, A. (2021) Separation and Preparative Purification of L‐and D‐valine Ester: Diastereomeric Conjugates of Atazanavir Using a Combination of 2‐propanol and Acetonitrile in Reversed‐phase High‐performance Liquid Chromatography. Sep. Sci. Plus, 4 (1), 16–23. https://doi.org/10.1002/sscp.202000046
  • Teja, P. K.; Mithiya, J.; Kate, A. S.; Bairwa, K.; Chauthe, S.K. (2021) Herbal Nanomedicines: Recent Advancements, Challenges, Opportunities and Regulatory Overview. Phytomedicine, 153890. https://doi.org/https://doi.org/10.1016/j.phymed.2021.153890
  • Teja, P. K.; Patel, P.; Bhavsar, D.; Bindusri, C.; Jadhav, K.; Chauthe, S.K. (2021) Traditional Uses, Phytochemistry, Pharmacology, Toxicology and Formulation Aspects of Glycosmis Species: A Systematic Review. Phytochemistry, 190, 112865. https://doi.org/https://doi.org/10.1016/j.phytochem.2021.112865
  • Asokan, K. and Shaikh, K.M. and Tele, S.S. and Chauthe, S.K. and Ansar, S. and Vetrichelvan, M. and Nimje, R. and Gupta, A. and Gupta, A.K. and Sarabu, R. and Wu, D.-R. and Mathur, A. and Bajpai, L., Applications of 2, 2, 2 trifluoroethanol as a versatile co-solvent in supercritical fluid chromatography for purification of unstable boronate esters, enhancing throughput, reducing epimerization and for additive free purifications, J. Chromato. A, 2018, 1531, 22-130.
  • Tiwari, R. and Ahire, D. and Kumar, H. and Sinha, S. and Chauthe, S.K. and Subramanian, M. and Iyer, R. and Sarabu, R. and Bajpai, L, Use of hybrid capillary tube setup on 400MHz NMR for quantitation of crucial low quantity metabolites using aSICCO signal, Drug Metab Dispos. 2017, 45, 1215-1224.
  • Laiakis, E.C.; Pannkuk, E.L.; Chauthe, S.K, Wang, Y.-W.; Lian, M. Mak, T.D.; Barker, C.A.; Astarita, G.; Fornace, A., A serum small molecule biosignature of radiation exposure from total body irradiated patients, J. Proteome Res., 2017, 16, 3805–3815.
  • Goudarzi, M.; Chauthe, S.K, Strawn, S.J.; Weber, W.M.; Brenner, D.J.; Fornace, A.J., Quantitative analysis of urinary citrulline and calcitroid acid in mice after external beam gamma-irradiation or exposure to internal emitters, Int. J. Mol. Sci. 2016, 17, 782.
  • Chauthe, S.K, Mahajan, S.; Rachamalla, M.; Tikoo, K.; Singh, I. P. Synthesis and evaluation of linear furanocoumarins as potential anti-breast and anti-prostate cancer agents, Med. Chem. Res. 2015, 24, 2476-84.
  • Chauthe, S.K, Bharate, S. B.; Periyasamy, G.; Khanna, A.; Bhutani, K. K.; Mishra, P. D.; Singh, I. P. One pot synthesis and anticancer activity of dimeric phlorolglucinols, Bioorg. Med. Chem. Lett. 2012, 22, 2251-2256.
  • Chauthe, S.K,; Sharma, R. J.; Gupta, R. C.; Singh, I. P. qNMR: An applicable method for standardization of Indian medicinal plants extracts and their herbal formulations, Phytochem. Anal.2012, 23, 689-96.
  • Chauthe, S.K, Bharate, S. B.; Sabde, S.; Mitra, D.; Bhutani, K. K.; Singh, I. P. Biomimetic synthesis and anti-HIV activity of dimeric phloroglucinols. Bioorg. Med. Chem. 2010, 18, 2029-2036.
  • Sabde, S.; Bodiwala, H. S.; Karmase, A.; Deshpande, P. J.; Kaur, A.; Ahmed, N.;Chauthe, S.K,Brahmbhatt, K. G.; Phadke, R. U.; Mitra D.; Bhutani, K. K. and Singh, I. P. Anti HIV activity of Indian medicinal plants, J. Nat. Med. 2011, 65, 662-669
  • Bharate, S. B.; Khan, S. I.; Yunus, N. A. M.; Chauthe, S.K, Jacob, M. R.; Tekwani, B. L.; Khan, A.; Singh, I. P. Antiprotozoal and antimicrobial activities of O-alkylated and formylatedacylphloroglucinols. Bioorg. Med. Chem. 2007, 15, 87–96.
  • Bharate S. B.; Chauthe, S.K, Bhutani K. K.; Singh I. P. An efficient two-step synthesis of jensenone. Synthesis of analogues and evaluation as antioxidants. Aust. J. Chem. 2005, 58, 7, 551-555.
  • Singh, I. P.; Chauthe, S.K, Small molecule HIV entry inhibitors-Part I: Chemokine receptor antagonists – 2004-2010, Expert Opin. Ther. Pat. 2011, 21, 227–269.
  • Singh, I. P.; Chauthe, S.K, Small molecule HIV entry inhibitors-Part II: Attachment and fusion inhibitors – 2004-2010, Expert Opin. Ther. Pat. 2011, 21, 399–416.

    • Natural Products Chemistry Lab

    Publications Abstracts

    Parusu Kavya Teja.; Bao Q. Ly.; Dhaval K. Shah.; Siddheshwar K. Chauthe. (2023). Isolation, Characterization of Undescribed Alkaloids, and Semisynthetic Modifications of Cytotoxic Pyranoacridone Alkaloids from Glycosmis pentaphylla, ACS Omega. doi.org/10.1021/acsomega.2c08100.

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    Research Scholars

    Student Details

    Ms. Parusu Kavya Teja

    Ms. Parusu Kavya Teja is pursuing her Ph. D. at the Dept. of Natural Products, NIPER-Ahmedabad. She received her M.S. (Pharm.) in Natural Products from NIPER-Kolkata in the year 2017-2019. During her master research programme, she had worked in CSIR-Indian Institute of Chemical Biology (IICB) on a project entitled ‘Exploring alpha-glucosidase inhibitory constituents from Elephant’s apple collected from North East India.’ under the supervision of Dr. Deepak Kumar, Scientist, CSIR-IICB. Her research interest includes isolation and characterization of bioactive compounds from medicinal plants and targeted semisynthetic modifications of isolated compounds for anti-cancer evaluation. She is also interested in formulation and development NDDS based herbal formulations. Currently, she is working on a project entitled ‘Bioprospecting, molecular docking analysis and synthetic modification of bioactive compounds from Glycosmis Pentaphylla for the discovery of potent anticancer compounds.’

    Mr. Suraj Kutre

    Mr. Suraj Kutre is pursuing his Ph. D. at Dept. of Natural products, NIPER-Ahmedabad. He received his M.S. Pharm in Natural Products from NIPER-Kolkata in the year 2019-2021. During his master research program, he had worked on a project entitled as ‘Qualitative and quantitative analysis of syringin, berberine , tinosporin, and isocolumbin in Tinospora cardifolia’ extract under the supervision of Dr. Satheesh nanjappan. Currently he is working on a project entitled as ‘Phytochemical investigations of Curcuma caesia for the potential anticancer and anti-inflammatory activity’ under Dr. Siddheshwar Kisan Chauthe, Assistant Professor, NIPER-Ahmedabad

    Ms. Sanjana kyada

    Ms. Sanjana kyada received her B. Pharm degree from noble Pharmacy College, Gujarat Technological University, Junagadh, India in the academic year 2021. Currently, she is pursuing M.S. Pharm in Natural Products, under the guidance of Dr. Siddheshwar Chauthe from the National Institute of Pharmaceutical Education and Research (NIPER), Ahmedabad, India. Her current research interest includes Isolation and characterization of novel compounds from fruits of Glycosmis pentaphylla as potential anticancer agents.

    Mr. Vaibhav Dattatray Kale

    Mr. Vaibhav Dattatray Kale received his B. pharm degree from S.M.B.T College of Pharmacy, Nashik affiliated to Savitribai Phule Pune University academic year 2021. Currently he is pursuing M.S Pharm in Natural Products, under the guidance of Dr. Siddheshwar Chauthe from National Institute of Pharmaceutical Education and Research (NIPER), Ahmedabad, India. His current research interest includes Isolation, characterization and enrichment of Anthocyanins from Rhododendron arboreum and development of anthocyanin enriched extract as a nutraceutical product.

    Ms. Rohini Ramesh Jadhav

    Ms. Rohini Ramesh Jadhav received her B. Pharm degree from MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik affiliated to Savitribai Phule Pune University in the academic year 2021. Currently she is pursuing M.S. (Pharm.) in Natural Products, under the guidance of Dr. Siddheshwar Chauthe from National Institute of Pharmaceutical Education and Research (NIPER), Ahmedabad, India. Her current research interest includes the Isolation and characterization of novel cytotoxic compounds from weed Tridax procumbens and evaluation of its in vitro anticancer activity

    Expertise

  • Natural products isolation and characterization using advanced analytical techniques.
  • Novel Herbal /Nutraceutical product development
  • Design and synthesis of analogues of bioactive natural products for various therapeutic activities
  • Well versed with structure elucidation of unknown compounds by 1D and 2D NMR techniques
  • Hands on experience on, HPLC, UPLC, SFC, Single Quad MS, triple quadrupole MS (Xevo-TQS), Ion trap, TOF-MS (Agilent-QTOF), Thermo-Orbitrap, Agilent HPLC/UPLC, Waters prep HPLC etc.
  • HPLC method development for achiral and chiral compounds for preparative purification of NCE’s.
  • MS/MS fragmentation study for structure confirmation of unknown compounds/process impurities by HRMS.
  • Mass spectrometric analysis of biomolecules (intact mass measurement, subunit protein analysis peptide mapping and antibody-drug conjugates).
  • SEC-HPLC, IEX-HPLC, Hydrophobic Interaction chromatography (HIC), RP chromatography for analysis of proteins/peptide
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